Product: Anti-C-Myc, Polyclonal
Cat. No: PC-217 (0.1 mg)
Procedure:
Chickens were immunized with the C-Myc
peptide conjugated with KLH. After multiple
immunizations in Freund’s adjuvant, eggs were
collected. Antibodies were immunoaffinitypurified
using the peptide immobilized on a solid
phase.
Specification:
ELISA: Antibody specificity was verified by
ELISA against the peptide conjugated to BSA. A
1:38,000 dilution of the antibody gave an OD =
1.0 in a 15 minute reaction using HRPconjugated
Goat anti-Chicken IgY (IgG) at
1:10,000 and TMB as the substrate. Appropriate
specificity controls were run.
Host: Chicken
Format:
Liquid. Affinity-purified antibody in PBS, pH 7.2
with 0.1% sodium azide.
Storage and Stability:
Store at +4°C.
Applications and Suggested Dilutions:

ELISA

Western blot: Use at ~1:5,000
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product