Chymotrypsinogen A (Bovine), 5x
产品名称： Chymotrypsinogen A (Bovine), 5x
英文名称： Chymotrypsinogen A (Bovine), 5x
产品编号： P5300
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Lyophilized. Dialyzed. Purified. 5X crystalline. Electrophoretically homogeneous. Intrinsic activity ≤ 0.25 units/mg chymotrypsin.
- Form:
- Lyophilized
- Preparation Method:
- Native protein purified from Bovine Pancreas
- Activity:
- Activates to at least 45 units per mg protein. One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density of 1330 N.F. units per mg using ATEE as a substrate (1 BTEE unit = 29.5 USP/NF units).
- Storage Instruction:
- Store at 4°C.
- Recommend Usage:
- Chymotrypsinogen A Assay
- Note:
- Chymotrypsinogen activation: Dissolve 100 mg enzyme in 5 ml reagent grade water. Adjust pH to 3.0 with 1 N H2SO4. Add 2.5 ml of 0.5 M phosphate buffer pH 7.6 and readjust pH to 7.6 if necessary. Add 1 ml (1 mg) trypsin and dilute to a final volume of 10 ml with reagent grade water.
- Biological function:
- The zymogen form of chymotrypsin. Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan.
- Application Image
- Enzyme Activity