创新点

•改进了上方荧光检测模块，支持包括CisBio HTRF在内的TR-FRET检测模式。
•新增的z轴聚焦功能有效降低上方荧光模式下的背景值，并能够针对不同微孔板实现自动优化测量，从而有效提高检测灵敏度。
•改进的底部荧光检测模式与多点测量功能令基于细胞的检测更为可靠。
•2OD减衰片取代1OD减衰片进一步扩展发光检测的动态范围。
•优化的加样器与试剂托架
•新增微孔板进出按钮

仪器介绍

仪器简介：

Tecan officially launched its new Infinite 200 Pro multimode Plate readers in both monochromator-based (M200 Pro) and filter based (F200 Pro) format today. The Infinite 200 Pro series replace its renowned Infinite 200 series, which were widely welcomed in the market since its release at 2005.

Many new features are implemented into the Infinite 200 PRO series, including:
• Enabled TR-FRET including CisBio HTRF certification
• New z-adjustment for FI top option with lower background and higher sensitivity
• Enhanced FI bottom with improved performance for cell based assays
• Extended dynamic range and improved sensitivity in luminescence mode
• Optimized injector handling for enhance convenience and better handling
• Built-in plate In/Out button for easy user
• And More…

Light source UV 
Xenon flashlamp

M200 PRO 
Quad4 Monochromators system

F200 PRO
Up to 4 filter pairs per slide

Wavelength range
FI Standard:
Ex 230 – 600 nm, Em 330 – 600 nm
Optional
Ex 230 – 850 nm, Em 280 – 850 nm
FP Standard
Ex 300 – 600 nm; Em 330 – 600 nm
Optional
Em 330 – 850 nm
Absorbance
230 – 1000 nm

Detectors
Fluorescence
PMT, optional UV and red-sensitive
Absorbance
UV silicon photodiode
Luminescence
photon counting system with low dark current PMT

Plate formats
6- to 384-well plates, cuvettes, NanoQuant Plate

Temperature control
Ambient +5 °C up to 42 °C

Shaking
Linear, orbital

Fluorescence Sensitivity*
Infinite F200 PRO
Fluorescence top reading*
85 amol / well (0,85 pM, 384-well plate)
Fluorescence bottom reading*
0.7 fmol / well (3,5 pM; 96-well plate)
TRF**
2.8 amol / well (28 fM; 384-well plate)
FP*
< 4 mP standard deviation @ 1nM Fluorescein
Infinite M200 PRO
Fluorescence top reading*
170 amol / well (1,7 pM; 384-well plate)
Fluorescence bottom reading*
1.2 fmol / well (6 pM; 96-well plate)
TRF**
90 amol / well (0.9 pM; 384-well plate)

Luminescence Sensitivity
Glow luminescence***
225 amol ATP / well (9 pM; low volume 384-well plate)
Flash luminescence****
12 amol ATP / well (218 fM; 384-well plate)

Absorbance
- Ratio accuracy 260 / 280 nm < ± 0.07
- Precision @ 260 nm < 0,2 %
- Accuracy @ 260 nm < 0,5 %
Measurement range 0 – 4 OD 

Injectors
Pump speed
100 – 300 μl/s, 1 μl increments;
max. volume
800 μl per stroke
Dead volume
100 μl including pump back

Fastest Read Times
96 well plate
20 sec
384 well plate
30 sec
Wavelength Ex / Em-scan
150 sec (96 well plate 450 – 550 nm, 5 nm step)


* Detection limit for Flourescein
** Detection limit for Europium
*** Detection limit for ATP (144-041 ATP detection kit SL (BioThema)
**** Detection for ATP (ENLITER Kit)

Fluorescence intensity top reading including
TRF, with automated z-adjustment
(optional background corrected z-focusing)
TR-FRET/HTRF®
Enhanced fluorescence intensity bottom reading
with OR (Optimal Read) function, including TRF
Spectrally enhanced photomultiplier tube
Absorbance
Photon counting luminescence,
including dual color luminescence
Cuvette port for absorbance
Temperature control
Injectors
NanoQuant Plate