名称：Blood & Cell Culture DNA Maxi Kit
产地：Qiagen
货号：13362
规格：10
价格：4120
For isolation of up to 500 µg high-molecular-weight DNA from blood ａnd cultured cells: Convenient, parallel processing of multiple samples. Reliable isolation of DNA up to 150 kb in size. No phenol or chloroform extractions.
Downstream applications
Analysis of gene targeting. Screening of transgenic animals. DNA fingerprinting studies. Southern blotting. QIAGEN Genomic-tips, included in Blood & Cell Culture DNA Kits, use unique QIAGEN anion-exchange technology to purify high-molecular-weight DNA from a wide range of biological samples without phenol or chloroform. PCR amplification. The QIAGEN Genomic-tip procedure is very gentle ａnd results in negligible DNA shearing. DNA purified with QIAGEN Genomic-tips is sized up to 150 kb with an average length of 50–100 kb.
Principle
The DNA is free of all contaminants such as RNA, protein, ａnd metabolites, ａnd has A260/A280 ratios between 1.7 ａnd 1.9, making it well suited for use in the following applications: RFLP analysis. Lysis buffers are optimized for different sample types ａnd provide immediate denaturation of proteins such as nucleases, histones, ａnd DNA-binding proteins, as well as potentially infectious viral particles. 
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