Invent Biotechnologies公司的Minute质膜蛋白分离试剂盒是由优化过的裂解液以及带2.0mL离心管的样品处理装置组成的。该试剂盒是用于从培养的哺乳动物细胞或组织中快速提取天然总膜蛋白（细胞器膜蛋白）以及天然质膜蛋白。该试剂盒最终提取到的4个部分为：细胞核，胞浆，细胞器蛋白以及质膜蛋白。由于使用了样品提取装置，所以质膜蛋白的提取简单，使用方便，并且能够得到很高的得率。与市面上其他提取膜蛋白试剂盒比较，该试剂盒并不需要大量的起始细胞（5x105以上）。该试剂盒所提供的起始细胞量范围广泛（1x104-1x105的样品范围）。该裂解液不含表面活性剂及EDTA。不需要使用匀浆器或组织研磨器。整个过程可以在45min内完成。

应用：

The kit is designed to rapidly isolate native membrane proteins from cultured cells or tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, membrane protein structure analysis, 2-D gels, enzyme activity assays and other applications. This kit provides the most rapid method currently available for preparation of native membrane proteins.

试剂盒组成：

1．25mL裂解液A

2．10mL裂解液B

3． 50个样品处理装置

4． 50个蛋白收集管

5． 4根样品处理棒

6． 组织解离珠

保存

将裂解液A和裂解液B都保存于-20℃

提示：

1. Read the entire procedures carefully. Thaw buffer A and buffer B completely, invert the bottles a few times and place them on ice. Chill protein extraction filter cartridge with collection tube on ice prior to use.

2. All centrifugation steps should be performed at 4oC in a cold room or in a refrigerated mirocentrifuge.

3. To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to buffer A prior to use. The use of protease inhibitor cocktails is optional.

4. It is recommended to use BCA Protein Assay Kit for determination of protein concentration (Pierce, Cat #:23227).